Powering High-Throughput Plant Genetics to Cultivate the Fruits and Vegetables of Tomorrow

Benchling provided a robust plant registry that tied actions like inventory tracking, data collection, and seed retrieval to the plant in question. This Registry provided a convenient system within which Pairwise could track their entire plant development pipeline. The pipeline began with an idea for a potentially beneficial gene edit. Researchers then designed plasmids and checked the gene edit’s sequencing results using Benchling Molecular Biology. Once a plasmid had been selected and validated, transformations into plants happened on a large scale. Templatized Benchling Notebook entries and workflows helped standardize data collection during large-scale gene editing and plant growth. As plants moved from the wet lab to the greenhouse, Benchling Requests provided a single communication point, ensuring that every handoff took place under identical conditions. Plants were molecularly screened using NGS, and seeds were only collected from those capable of passing on the desired trait. The subsequent generation was then screened to confirm the trait had been inherited. Benchling associated each plant’s database entry with these experimental results, making it easy to identify candidate plants and seeds, and discard undesirable ones. API access allowed Pairwise to pull data directly from specialized instruments and to build customized report views.