Case Studies
A High-Dimensional Space-filling DOE for Assay Development
Overview
Analytics & Modeling - Predictive Analytics Application Infrastructure & Middleware - Data Visualization Functional Applications - Remote Monitoring & Control Systems | |
Pharmaceuticals Life Sciences | |
Product Research & Development Quality Assurance | |
Predictive Maintenance Process Control & Optimization Digital Twin | |
System Integration Software Design & Engineering Services | |
Operational Impact
The combination of SPT Labtech’s dragonfly® discovery dispenser with Synthace allowed for the characterization of a spectrophotometric enzymatic assay using a space-filling DoE with a total of 3,456 runs for three sets of triplicates of 384 runs and the corresponding controls. | |
Synthace and dragonfly® discovery combine to create an efficient and robust solution to reduce assay development cycles with automated DOE execution and data aggregation. | |
This approach can prevent future bottlenecks in assay transfer by enabling digitization of assay protocols and use of the same liquid handler from assay development through to high-throughput screening, reducing the need to miniaturize or re-optimize assays when proceeding into HTS campaigns. | |
Quantitative Benefit
Synthace reduced time spent on planning the 20,745 liquid handling steps and data aggregation from the microplate reader by 75% and 94% respectively. | |
The dragonfly® discovery reduced liquid handling time by 98% compared with an average pipetting robot. | |
Eliminated 99.9% of the number of tips needed. | |